Determine if you will use a stovetop or microwave to heat the water. Ill also provide the approximate length of each unit. After conducting this lab it was determined that primary productivity can indeed be measured using dissolved oxygen levels. Use the explanation for each section as a guideline. The faster the water moves, the more dissolved oxygen the water will contain since it has more contact time with the air. Distilled water contains no organisms, is stagnant, and is kept at room temperature. To determine whether the temperature of the water impacts the rate in which oxygen is dissolved in water hypothesis. Dissolved oxygen and aquatic primary productivity measure amount of o2 consumed dissolved oxygen do after exposure to light or darkness by titration difference between initial and light net productivity photosynthesis increases do. At 20c oxygen diffuses 300,000 times faster in air than in water, making the distribution of oxygen in air relatively uniform. It also demonstrated the effect of light and nutrients on photosynthesis. Information about dissolved oxygen learn with flashcards, games, and more for free. The light oxygen has 16 mg dissolved oxygen and then subtracted to 6 mg dissolved oxygen so that equals to 10 mg dissolved oxygen by net productivity. Biology with vernier addresses the fundamentals of a high school or college general biology course with 31 experiments that include cell respiration, photosynthesis, membrane diffusion, osmosis, human physiology, transpiration, fermentation, and more.
Choose from our kits, follow a college board lab, or design your own with our wide variety of equipment and supplies. These all contribute to a lower dissolved oxygen concentration. As water becomes warmer, its ability to hold oxygen decreases. Dissolved oxygenclassic lab kit for ap biology, 8 groups. Andersen demonstrates the azidewinkler method of dissolved oxygen analysis. In the dissolved oxygen classic lab kit for ap biology, the relationship between the amount of do in water and water quality are explored and quantified using the winkler method. Chemicals for the winkler technique are provided in groupsized increments. In the first part of the lab, students learn how to measure the rate of photosynthesis. Spatial distribution of oxygen in water, on the other hand, can.
This form of oxygen can fit into the spaces between water molecules and is available to aquatic organisms. Animal behavior paul andersen introduces the concept of ethology and contrasts kinesis and taxis. The difference in the dissolved oxygen levels between the uncovered tube and the covered tube is the biochemical oxygen demand of the water sample. Ap biology 2008 scoring guidelines form b question 1 1. It further solidifies the argument that ap biology students would benefit from taking a good. Andersen demonstrates the azidewinkler method of dissolved oxygen. Measurements of dissolved oxygen do are used to determine primary productivity in bodies of water. Since oxygen is one of the most easily measured products of both photosynthesis and respiration, a good way to gauge primary productivity in an aquatic ecosystem is to measure dissolved oxygen. This lab book provides instructions for data collection with logger pro 3 and labquest app only.
You have remained in right site to begin getting this info. Explain the relationship of dissolved oxygen to primary productivity. The silver ring on the probe should be immersed in the culture, but the probe should not be touching the bottom of the beaker. Wave action, waterfalls and rapids aerate water an increase oxygen concentration. Dissolved oxygen and aquatic primary productivity, answer the following questions before midnight the day before the lab or you can not do the lab. In this portion of the laboratory you monitor the effect of varying light levels on dissolved oxygen in an algaerich water culture. If the temperature of the water is increased, then there is no effect on the rate of dissolution of oxygen inside water iv.
Oxygen from the atmosphere is mixed into the water through diffusion. Videos anatomy and physiology ap biology ap chemistry. Dissolved oxygen and primary productivity lab savage science. Dissolved oxygen prentice hall ap bio lab 12 lab bench quiz. You can save time by assigning each group one temperature of water for do determination and taking class averages. Ap biology lab manual for teachers revised edition by college board author isbn. This book contains 18 water quality tests, including ph, total dissolved solids, dissolved oxygen, bod, flow rate, turbidity, nitrates, and phosphates. Effects of temperature, dissolved oxygen, salinity and ph. Ecology, experimental design, microbiology, lab skills. In conclusion, an increase in temperature and an increase in light increase the amount of dissolved oxygen within a water sample. In aquatic environments oxygen production and oxygen usage must be balanced to prevent anoxia. Measure the amount of dissolved oxygen in water samples at different temperatures 2. Includes instructions on water sampling, an illustrated.
Effect of temperature on dissolved oxygen we actually did this part of the lab already. Andersen walks you through the cellular respiration lab. Ap biology lab manual for teachers supplement technology integration use of gas pressure probeware provides a different means of running lab 2 a more rapidly, b without the necessity of using caustic sulfuric acid or messy permanganate stain and c without timing problems involved in stopping the reaction at specific points. Labs demo labs should be legibly written in student composition books following the same general format. But the additional exaggeration is by collecting the soft file of the book. Dissolved oxygen and aquatic primary productivity kit. Analyze the effect of varying amounts of light on primary productivity. If npp for an ecosystem is positive, what will happen to the biomass of that ecosystem over time. Now, in your own words, explain why this calculation works. At 4,000 meters in elevation,000 feet, the amount of dissolved oxygen in water is less than twothirds what it is at sea level.
The concentration of dissolved oxygen in water depends also on temperature, pressure, and concentrations of various ions cf. Oxygen is used by organisms in aerobic respiration, where energy is released by the combustion of sugar in the mitochondria. Alignment to the ap biology curriculum framework this investigation can be conducted during the study of concepts pertaining to cellular processes big idea 2, specifically, the capture, use, and storage of free energy, or interactions big idea 4. However, more oxygen is mixed into the water with the help of winds, rain, waves, and currents. Permission granted to make unlimited copies for use in any one school building. Dissolved oxygen lab answers dissolved oxygen lab answers recognizing the quirk ways to get this book dissolved oxygen lab answers is additionally useful. Climate change impacts, vulnerabilities and opportunities analysis of the. I have done it, but the new manual is certainly an improvement over the old procedure. The dissolved oxygen probe is great for biology, chemistry, ecology, or integrated science courses. In exercise 12a you will measure and analyze the dissolved oxygen do concentration in water samples at varying temperatures. Dissolved oxygen lab answers dissolved oxygen lab answers getting the books dissolved oxygen lab answers now is not type of inspiring means. Prepare your students for medical and lab tech careers with carolina s wide range of equipment, kits and models. Ap biology course outline 20092010 rock creek schools.
Dissolved oxygen and aquatic primary productivity introduction in an aquatic environment, oxygen must be dissolved in order to be available for use by organisms. This manual contains background information, lesson ideas, procedures. Prepare the setups according to how you schedule the activities. Clearly explain the difference between gross primary productivity gpp and net primary productivity npp 1b. Students should develop the skills necessary to implement the selected procedure. You could not and noone else going later ebook buildup or library or borrowing from your connections to entre them.
The 8station kit includes enough materials for a class of 32 students working in groups of 4. Start a free trial of quizlet plus by thanksgiving lock in 50% off all year try it. We also predict that all of our experimental variables will contain more dissolved oxygen than our control, the distilled water. We have kits for new and traditional ap biology labs. Print out the completed calculation table from sample problem page of the labbench web site. By engaging with these educational materials, students learn. In exercise 12b you will measure and analyze the primary productivity of. Remind yourself of our experiment, our procedures, and our conclusions. Safe and compact titration assemblies providedselect from an extensive catalog of educational tools available for enhancing the teaching of biological subjects, including simulated testing of blood and urine, the illustration of various natural habitats, and the dissection of sterilized stool samples collected from different regions. What does it take to make the dissolved oxygen lab successful. Part b of the lab was used to measure dissolved oxygen concentration, gross and net productivity, and respiration rate of the water samples.
The test results indicated that after a 24hour time period, there was a difference in the primary productivity relative the amount of light tested. Measuring dissolved oxygen do accurately is very difficult but students should attempt it anyway. We were investigating the effect of the physical factor of. I compared it to a chart of 100 percent oxygen solubility at different temperatures, and in all cases for the chart values at 100 percent, the nomograph reads. All students enrolled in this course are expected to take the ap biology exam. We cannot measure gross productivity directly because respiration, which uses up oxygen and organic compounds, is always occurring simultaneously with. Remove the do probe from the storage bottle and carefully insert it into the culture. Seeing that the results were consistent through multiple experiments, it is highly likely that if the experiment was repeated that similar or the same results would occur. The instructor information section included for each experiment contains tips for successful completion, reagent. In an aquatic environment, oxygen must be dissolved in order to be available for use by organisms. Dissolved oxygen and primary productivity 6 for evaluation purposes only 39. Temperature and dissolved oxygen the advanced placement biology laboratory manual recommends that each student group determine do of water samples at three different temperatures.
Dissolved oxygen and primary aquatic productivity laboratory 12 introduction dissolved oxygen levels are an extremely important factor in determining the quality of an aquatic environment. Although water is composed of oxygen and hydrogen atoms, biological life in water depends upon another form of oxygenmolecular oxygen. For example, if you follow the schedule suggested in time requirements, above, on lab day 1 students will need all the materials listed for exercise 2a plus 15 ml of h 2o 2 in a 60ml cup labeled h 2 o 2. Dissolved oxygen in an aquatic environment, oxygen must be in a solution in a free state o 2 before it is available for use by organisms bioavailable. Dissolved oxygen lesson plan template and teaching resources. The concentration of the oxygen in aquatic environments is a very important component of water quality. File type pdf ap biology lab 11 answers ap biology lab 11 answers ap biology lab 11. Create a hot water bath by performing the following steps. Ap biology lab manual for teachers supplement when burettes are not available, titration can be done with droppers counting drops or with syringes which can easily measure mls. Neither the student book nor the teachers guide is clear on this. Measure the amount of dissolved oxygen in water samples at different temperatures lab 12.
In bright light, aquatic plants are able to release more oxygen. Measure the amount of dissolved oxygen in water samples at different temperatures. It can be used to perform a wide variety of experiments to. Ap and advanced placement program are registered trademarks of the. Ap sample lab 12 dissolved oxygen biology junction. To be successful, a method for measuring dissolved oxygen must meet two requirements. Terrestrial environments hold over 95% more oxygen than aquatic environments. While i was recently going over the dissolved oxygen data from lab 12, i realized that the nomograph in edition d page 140 of the ap lab manual is very poor and inaccurate. All of these physical factors, along with oxygen concentration, work together to increase diversity in aquatic habitats. The advanced placement biology laboratory manual recommends that each student group determine do of. Dissolved oxygen is necessary for the metabolic processes of almost every organism. In addition, some questions students are likely to raise connect to evolution big idea 1. We were investigating the effect of the physical factor of temperature on the percent dissolved oxygen in a body of water. Authors dean goodwin has been involved in designing and implementing environmental curricula since the late 1980s and is currently the director of environmental education at kimball union academy in.